Browsing by Subject "10-Gingerol | 6-Gingerol | 6-Shagoal | HPLC | zingiber officinale"
  • Publication
    Quantitative analysis method for zingiber officinale water extract using high-performance liquid chromatography
    ( 2024-01-01)
    Mohd Sharizan Md Sarip
    ;
    Nik Daud N.M.A.
    ;
    Mohd Zainudin M.A.
    ;
    Ibrahim L.H.
    ;
    Syahrul Affandi Saidi
    ;
    Idham Z.
    ;
    Anuar A.
    Quantitative analysis of the Zingiber Officinale sample using subcritical water extraction (SWE) was developed employing High-Performance Liquid Chromatography (HPLC) to bolster the advancement of this innovative green extraction process. This research focuses on three principal ginger bioactive compounds: 6-gingerol, 6-shagoal, and 10-gingerol. Various stages were undertaken to establish the quantitative analysis method, encompassing the optimisation of HPLC operating conditions and the formulation of standard calibration curves, yielding individual compound equations. A robust correlation within the calibration curve was achieved, exhibiting an r2 value of 0.9814 and an RSD of 5.00%. A simultaneous, swift, and dependable method was established with an injection time of 20 minutes and an 8-minute delay between injections, in contrast to the previous HPLC analysis requiring a 45-minute injection time for detecting and quantifying all components. Notably, no post-treatment was applied after the SWE process. This advancement allows for potential future online measurement of Zingiber Officinale bioactive compounds extracted using subcritical water extraction through this technology.