Low power electrical output detection based electronic readers are the key factor for biosensor commercialization. Generally, two-electrode based amperometric biosensors show extremely low current signal output around picoampere (pA) to microampere (μA) range. In this research, a portable electronic reader system with I/V converter amplifier was developed to detect current output values for two-electrode amperometric biosensors in selectable ranges of pA to nA. The system consists of commercially available two inverting amplifiers Burr-Brown OPA 129, microcontroller unit (MCU), 16-bit I2C, extra component for input/output protection, and power supply unit. The Arduino software and Proteus software were used to design and simulate the system properly before implement the hardware part. The complete system was verified via testing with variable high-impedance current source as input and compared the experimental result with theoretical output. The range of the current measurement of the system was from 1 pA to 250 nA for two-electrode based biosensors.
