Publication:
Effect of amino acid concentration on the gold nanoparticle for Calcium Phosphate formation UV-Vis and SEM studies
Effect of amino acid concentration on the gold nanoparticle for Calcium Phosphate formation UV-Vis and SEM studies
Date
2022-12
Authors
Annisa Tsalsabila
Yuliati Herbani
Yessie Widya Sari
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Abstract
The strong binding of amine to gold nanoparticles increases the usage of amino acids to functionalize gold nanoparticles. In this study, different charged amino acids were used as a capping agent for the gold nanoparticle to induce calcium phosphate formation. Glutamic and aspartic acid were negatively charged, while lysine and arginine were used as positively charged amino acids. The final ratio of gold to amino acid concentration (𝑐𝐴𝑢⁄𝑐𝐴𝐴) was 0.6 and 0.45. The variation concentration effect of amino acids in binding with gold nanoparticles was characterized using ultraviolet-visible (UV-Vis) spectroscopy. Slightly shifted in UV-Vis absorbance was recorded after gold nanoparticles were functionalized by amino acids indicating the existence of amino acids on gold nanoparticle surfaces. After being capped with amino acids, the solution of gold nanoparticles was tested using zeta potential, which showed the high stability of the solutions containing aspartic acid. Finally, the amino acid-capped gold nanoparticles solution was added by calcium and phosphate sources to form calcium phosphate. Interestingly, not all samples showed the existence of calcium phosphate formation based on scanning electron microscopy-energy-dispersive Xray spectroscopy (SEM-EDS) results. However, the EDS results indicate that calcium phosphate was formed on the sample that contained lysine with 0.45. The results suggest that the calcium phosphate formation on amino acid-capped gold nanoparticles depends on the amino acid and the ratio concentration of gold nanoparticles to amino acids.
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Keywords
Amino acids,
Calcium phosphate,
Gold nanoparticles