Nur Farahiyah Mohammad
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Nur Farahiyah Mohammad
Official Name
Nur Farahiyah, Mohammad
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Mohammad, N. F.
Nur Farahiyah, Mohammad
Mohammad, N.
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Scopus Author ID
36872346600
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X-6539-2019

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The total phenolic content, total flavonoid content and antioxidant properties of E.tirucalli L. extract partitioned using different solvents

2025 , Mangalagowri Sangar , Nur Farahiyah Mohammad , Siti Shuhadah Md Saleh , Alwani Ibrahim , Farah Diana Mohd Daud , Roy Francis Navea , Khairul Farihan Kasim , Nashrul Fazli Mohd Nasir

E.tirucalli L. is a succulent plant native to Africa renowned for its medicinal properties and antioxidant activity. This study aimed to evaluate the total phenolic and flavonoid content as well as the antioxidant activity of E.tirucalli L. extracts obtained using different solvents. Aqueous ethanol, hexane, and dichloromethane were used for extraction through Soxhlet extraction and partitioning. The total phenolic content was highest in the aqueous ethanol extract (2.501 mg GAE/g), followed by hexane (0.110 mg GAE/g) and dichloromethane (0.050 mg GAE/g) extracts. Similarly, the total flavonoid content was highest in the aqueous ethanol extract (1.307 mg QE/g), followed by hexane (0.164 mg QE/g) and dichloromethane (0.061 mg QE/g) extracts. The DPPH assay demonstrated that the aqueous ethanol extract exhibited the highest radical scavenging activity (RSA) with an IC50 value of 36.89 ± 0.05 μg/mL, followed by dichloromethane (50.94 ± 0.39 μg/mL) and hexane (62.42 ± 1.34 μg/mL) extracts. These findings indicate that aqueous ethanol is an effective solvent for extracting phenolic and flavonoid compounds with potent antioxidant activity from E.tirucalli L. extracts.

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Quantification of polyphenols content and antioxidant activity of Euphorbia Tirucalli l. extracted using Maceration and Soxhlet method

2025 , Alwani Ibrahim , Nur Farahiyah Mohammad , Siti Shuhadah Md Saleh , Nashrul Fazli Mohd Nasir , Khairul Farihan Kasim , Mangalagowri Sangar , Farah Diana Mohd Daud , Roy Francis Navea

Euphorbia tirucalli L. (E. tirucalli) has gained attention for these past few years in terms of its phytochemical studies due to the antioxidant attribution of polyphenols source as its bioactive compounds in extracts. In order to extract important plant compounds, conventional extraction methods such as maceration and Soxhlet are still widely incorporated for plant extraction techniques due to their convenient application in laboratory settings. This study focused on the two conventional techniques; maceration and Soxhlet on polyphenols extraction of E. tirucalli using various extraction solvents. In order to provide insight into its potential for polyphenols extraction, yield percentage (%) and phytochemical tests such as Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and antioxidant activity (%) were assessed. In terms of yield percentage, Soxhlet methanolic extract was found to be the best. The findings also suggest that both maceration and Soxhlet techniques are effective in extracting a significant amount of phenolics and flavonoid content in which highest value recorded were 17.26 ± 0.23 mgGAE/100g and 50.08 ± 1.13 mgQE/100g for maceration while 16.17 ± 0.21 mgGAE/100g and 43.02 ± 0.01 mgQE/100g for Soxhlet. Maceration with methanol solvent appears to be more effective for TPC and TFC while Soxhlet acetonic extract is chosen as the best for antioxidant activity with 75 .79 ± 0.04 % of radical scavenging activity in E. tirucalli extract. Thus, this study indicates the noteworthy potential of both maceration and Soxhlet techniques for polyphenols extraction.

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