Nor Azizah Parmin
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Nor Azizah Parmin
Official Name
Nor Azizah, Parmin
Alternative Name
Parmin, N. A.
Parmin, Nor Azizah
Parmin, Nor A.
Main Affiliation
Scopus Author ID
57195835481
Researcher ID
S-6303-2019

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  • Publication
    Gold Nanoparticles Enhanced Electrochemical Impedance Sensor (EIS) for Human Papillomavirus (HPV) 16 Detection E6 region
    ( 2020-07-09)
    Amrul Muhadi A.S.
    ;
    Nor Azizah Parmin
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Muhammad Nur Afnan Uda
    ;
    Muhammad Nur Aiman Uda
    ;
    Hong V.C.
    The persistent infection by high risk HPV is a necessary but not sufficient cause of this cancer which develops over a long period through precursor lesions which can be detected by electrochemical impedance sensor. The HPV driven molecular mechanisms underlying the development of cervical lesions have provided a number of potential biomarkers for both diagnostic and prognostic use in the clinical management of women with HPV related cervical disease and these biomarkers can also be used to increase the positive predictive value of current methods. The most influential methods for the detection and identification of HPV using gold nanoparticle (GNP) included electrochemical impedance sensor will visit their sensitivity, selectivity and characteristic detection on synthetic target which are complement of the DNA, non-complement of the DNA and mismatch of the DNA. In difference concentration of synthetic target, which stage can get the exactly value to determine the HPV in strain 16 was evaluated in this research studies.
  • Publication
    Voltammetric DNA Biosensor for Human Papillomavirus (HPV) Strain 18 Detection
    ( 2020-07-09)
    Mhd Akhir M.A.
    ;
    Nor Azizah Parmin
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    ;
    Voon Chun Hong
    This research was developed to focus on the study of the voltammetric DNA biosensor for the detection of HPV strain 18. In this research, electrical DNA biosensor was expected to detect HPV strain 18 more efficiently by using electrical characterization. In this project, device inspection was conducted to make sure the functional of the gold interdigitated electrode (IDE) by using Scanning Electron Microscope (SEM). 3-Aminopropyl Triethoxysilane (APTES) solution was used for the process of surface modification to form the amine group on the surface of the device to facilitate the attachment of the DNA probe. In this project, synthetic DNA sample and DNA from the saliva of several Biosystems Engineering students were used as the target DNA. The current-voltage (I-V) electrical characterization was conducted to detect the presence of HPV strain 18 in both DNA samples. As the results, perfect alignment between the electrodes on the IDE was detected under SEM. Surface modification of the biosensor successfully conducted which is the covalent bond between APTES and DNA probe increase the electrical. Synthetic DNA shows the presence of HPV strain 18 while there was no HPV strain 18 detected in the DNA from saliva samples.
  • Publication
    Novelty Studies on Amorphous Silica Nanoparticle Production from Rice Straw Ash
    ( 2020-07-09)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Halim N.H.
    ;
    Anbu P.
    Turning waste product into the valuable resources is the best alternative way to overcome the waste management issue. Generally, rice is grown and planted twice a year where a lot of rice by-products have been produced after harvesting the matured paddy. Rice straw is one of turning waste products into the valuable resources and to manage the environmental issues. Generally, rice is grown and planted twice a year where a lot of rice by-products are produced. Rices straw is one of the rice by-products, generated roughly 0.7-1.4 kg per kilograms of harvested milled rice. With the nanotechnological approach, silica particles at nano-size can be produced using the incinerated rice straw. In addition to that, this research will report the synthesis, characterization and adsorption analysis towards the heavy metal removal.
  • Publication
    Designing specific SARS-CoV-2 DNA probe as biosensing element for development of biosensor
    ( 2024-03-21)
    Halim F.S.
    ;
    Nor Azizah Parmin
    ;
    Uda Hashim
    ;
    Farrah Aini Dahalan
    ;
    Zakaria I.I.
    ;
    Subash Chandra Bose Gopinath
    ;
    Ang W.C.
    ;
    Jaapar N.F.
    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a part of the family of beta-coronaviruses inducing COVID-19 disease. COVID-19 became the most life-threatening and highly contagious viral disease compare to another disease family of coronavirus. Right now, the RT-PCR is the gold standard for the diagnostic of COVID-19. To produce successful prevention strategies, medical testing, vaccinations, and antiviral drugs against COVID-19, comprehensive biological information is critical. Conserved coding RNA sequence within the N gene region of the open reading frame in the SARS-CoV-2 genome was employed as the foundation for creating an oligonucleotide probe as it is a crucial component for the development of a biosensor to identify the virus. The study aimed to develop a DNA probe that complementary to the RNA target of the N region for SARS-CoV-2. Studies were conducted on various strains of coronavirus sequences to verify the percent of correlation as well as the region of consensus that triggers various strains of viruses. Basic local alignment search tools (BLAST) and CLUSTLW had devoted additional statistical parameters, for example, desire values (E-values) and score bits. The 30 mer DNA probe with 50.0% of GC content was developed, CTG AAG CGC TGG GGG CAA ATT GTG CAA TTT. The adequate length of the probe is between 22 and 31 mer. The complementary DNA probe was designed based on the RNA target from the N-region selection that has been identified that could be used as a biomarker probe to produce a biosensor that can be implemented to the clinical and environmental diagnosis of COVID-19.
  • Publication
    Evaluation and Optimization of Genomic DNA Extraction from Food Sample for Microfluidic Purpose
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Shaharuddin S.N.A.
    Contamination of various food samples became one of the critical issues in food pathogen infection. Food pathogen can be detected by using digital polymerase chain reaction (PCR) and sequencing. These methods were reliable but consuming and take a longer time for detection. The present work describes the innovation to develop a technology to extract double-stranded deoxyribonucleic acid (dsDNA) from food samples and then denatured dsDNA into and single-strand DNA (ssDNA) for further use on the chip using microfluidic device. Microfluidic device is a lab-on-chip device that consist of microfluidic channels that provide paths for biomolecules to flow to individual point of care. DNA extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. Lysis solution is used in the process of extraction the DNA to break up the cells containing DNA from protein and other cellular materials. This extraction firstly be done in the most labour-intensive in obtaining the DNA biomolecules. Extraction methods may require an overnight incubation, may be a protocol that can be completed in minutes or a couple of hours by using a commercial kit. The disadvantages of the laboratory and commercial kit is due to time-consuming, poor cost-effectiveness, the need to use big laboratory and a complicated process which need an expertise to conduct the experiment and interpret the data. This research is proposed to design and fabricate a microfluidic device that has DNA extraction capabilities. In this research DNA extraction using a commercial kit will be used as a comparison for the quality of the result. The microfluidic device can be used in health care delivery system and will help the doctors in diagnostic process to identify disease of a patient rapidly. Other than that, the output extracted from microfluidic device will be used for DNA probe target interaction for diagnostic kit. The major advantage of microfluidic device is that it consumes less time compared to the conventional chemical methods.
  • Publication
    Simple and Green Approach Strategy to Synthesis Graphene Using Rice Straw Ash
    ( 2020-07-09)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    ;
    Nur Hulwani Ibrahim
    ;
    Nor Azizah Parmin
    ;
    Halim N.H.
    ;
    Anbu P.
    Abundant resources of rice straw and carbon-neutral have a lot of potentials to be converted into various value-added product and become more green due to less utilization of chemical usage. Graphene is one of value-added had been converted from rice straw and was successfully synthesized by using activating rice straw ash (RSA) using potassium hydroxide (KOH) at 700 oC with 1:2.5 impregnation ratio. scanning electron microscopy (SEM), field emission scanning electron microscopy (FESEM), EDX Analysis with a field emission transmission electron Microscope (FETEM) and atomic force microscopy (AFM). The current results can be the model for comparing the synthesize graphene from other plant sources.
      1
  • Publication
    Label-free aptamer based biosensor for heavy metal detection
    ( 2020-11-02)
    Muhammad Nur Aiman Uda
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Nor Azizah Parmin
    ;
    Muhammad Nur Afnan Uda
    ;
    Mohammad Isa Ahmad Azan
    Heavy metal contamination such as arsenic becomes serious threat to the health and environment of many millions in a worldwide. Arsenic contamination especially in paddy field has driven to the accumulation of As in paddy soils and possibly have adverse effects on rice products. To assess the level of severity of arsenic in paddy field is a major constraint at early stages. In the past, quantification of concentration of arsenic has been as difficult as reducing it. Thus label free Aptamer based biosensor is the most reliable sensor recently due to the advantages for the sensitive and selective detection towards detecting arsenic. Finally, this paper will discuss the current outcomes in arsenic detection using Aptasensor with sensing element formatting in enhancing the sensor signal for detection.
      3  16
  • Publication
    Effect of Aeration Rate on Specific Oxygen Uptake Rate (SOUR) in Treating Chemical Oxygen Demand (COD) in Domestic Wastewater
    ( 2024-01-01)
    Zubir A.A.A.
    ;
    Farrah Aini Dahalan
    ;
    Kamarudin N.S.
    ;
    Naimah Ibrahim
    ;
    Ong Soon An
    ;
    Nabilah Aminah Lutpi
    ;
    Masitah Hasan
    ;
    Nor Azizah Parmin
    Specific oxygen uptake rate (SOUR) is significant parameter to determine the microbial activity and examined the effluent quality in biological wastewater treatment. Chemical oxygen demand (COD) is the major indicator in monitoring the effluent quality in relation on its removal mainly depends on the microbial activity in the activated sludge. So, this research is conducted to study the effect of aeration rate on SOUR and determined the best oxygen requirement in removing COD in domestic wastewater. The procedure was carried out by using domestic wastewater as the seed sludge in sequencing batch reactor. The reactor with working volume of 2L was operating 6 cycles in 24 hours with five phases (feeding, aeration, settle, draw and idle). The aeration time is fixed to 2.5 hours. The dissolved oxygen and COD readings were recorded with four types of aeration rate adjusted at 1L, 2L, 3L and 4L / min daily for 7 days. The result indicates that, 3L/min gives the highest SOUR which reflects that the high activity of microbial in this condition. Besides, the effluent also shows the highest COD removal efficiency on 3L/min of aeration rate. So, as a conclusion the best oxygen requirement for the microbial to carry out their activities on aeration rate of 3L/min.
      10  30
  • Publication
    Design 5.0 µm Gap Aluminium Interdigitated Electrode for Sensitive pH Detection
    ( 2020-07-09)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Nor Azizah Parmin
    ;
    Bakar A.H.A.
    ;
    Anuar A.
    ;
    Bakar M.A.A.
    ;
    Sulaiman M.K.
    The aim of the research study to design high sensitive biosensor for medical applications. IDE pattern was designed using AutoCAD software with 5 µm ginger gap. The fabrication process was done using a conventional photolithography process and standard CMOS process. The fabricated electrode was physically characterized using a low power microscope (LPM) and a high power microscope (HPM). The electrically validated through I-V measurements and chemically tested with different pH buffer solutions. Al IDE was well fabricated with 0.1 µm tolerance between the design mask and fabricated IDEs. Electrical measurements confirmed that IDE was well fabricated without any shortage and results of similar IDE samples were confirmed that the repeatability of the device. The extremely small current variations in nano ampere range were quantitatively detected using an extra small volume of 2 µl for different pH buffer solutions. It is confirmed that IDEs are sensitive in both alkali and hydroxyl ions medium.
      4  7
  • Publication
    New Development Quantification Methods for Salt Iodine and Urinary Iodine Using Microfluidics Based Nanotechnology
    ( 2020-03-18)
    Nur Hulwani Ibrahim
    ;
    Midhat Nabil Ahmad Salimi
    ;
    Muhammad Nur Aiman Uda
    ;
    Nor Azizah Parmin
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    In Malaysia, the first Iodine Deficiency Disorders (IDD) survey was conducted in 1996 and it was discovered that Peninsular Malaysia did not have IDD problem until latter studies showed goitre occurrence of 34.7% in Hulu Langat district and urinary iodine lower than the adequate level of (100-199 ug/L) in Perak and Pahang states (Selamat et al., 2010). Baseline and periodical sampling of children and pregnant woman urine and imported salt commodities for the consumption of the population is mandatory for iodine measurement. Thus, development of quantitative methods of measurement of salt and food iodine is crucial for implementation of the USI program nationwide. In this study, interdigitated electrode (IDE) biosensor, a rapid, sensitive and selective method has been developed to determine the iodine content in both urine and salt. This method includes functionalization and silanization step using 3-aminopropyl triethoxysilane (APTES). The I-V characterization of IDE biosensor was performed using (Keithley 2450), Kickstart software and Probestation. It measures the amount of current flow through IDE which is directly proportional to the concentration of iodine in both urine and salt. Hence, IDE biosensor is proven to be a rapid, selective, sensitive method and can be developed as a new nanotechnology for the elimination of Iodine Deficiency Disorders (IDD) among children and pregnant woman.
      32  1