Nor Azizah Parmin
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Nor Azizah Parmin
Official Name
Nor Azizah, Parmin
Alternative Name
Parmin, N. A.
Parmin, Nor Azizah
Parmin, Nor A.
Main Affiliation
Scopus Author ID
57195835481
Researcher ID
S-6303-2019

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  • Publication
    New Development Quantification Methods for Salt Iodine and Urinary Iodine Using Microfluidics Based Nanotechnology
    ( 2020-03-18)
    Nur Hulwani Ibrahim
    ;
    Midhat Nabil Ahmad Salimi
    ;
    Muhammad Nur Aiman Uda
    ;
    Nor Azizah Parmin
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    In Malaysia, the first Iodine Deficiency Disorders (IDD) survey was conducted in 1996 and it was discovered that Peninsular Malaysia did not have IDD problem until latter studies showed goitre occurrence of 34.7% in Hulu Langat district and urinary iodine lower than the adequate level of (100-199 ug/L) in Perak and Pahang states (Selamat et al., 2010). Baseline and periodical sampling of children and pregnant woman urine and imported salt commodities for the consumption of the population is mandatory for iodine measurement. Thus, development of quantitative methods of measurement of salt and food iodine is crucial for implementation of the USI program nationwide. In this study, interdigitated electrode (IDE) biosensor, a rapid, sensitive and selective method has been developed to determine the iodine content in both urine and salt. This method includes functionalization and silanization step using 3-aminopropyl triethoxysilane (APTES). The I-V characterization of IDE biosensor was performed using (Keithley 2450), Kickstart software and Probestation. It measures the amount of current flow through IDE which is directly proportional to the concentration of iodine in both urine and salt. Hence, IDE biosensor is proven to be a rapid, selective, sensitive method and can be developed as a new nanotechnology for the elimination of Iodine Deficiency Disorders (IDD) among children and pregnant woman.
  • Publication
    Gold Nanoparticles Enhanced Electrochemical Impedance Sensor (EIS) for Human Papillomavirus (HPV) 16 Detection E6 region
    ( 2020-07-09)
    Amrul Muhadi A.S.
    ;
    Nor Azizah Parmin
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Muhammad Nur Afnan Uda
    ;
    Muhammad Nur Aiman Uda
    ;
    Hong V.C.
    The persistent infection by high risk HPV is a necessary but not sufficient cause of this cancer which develops over a long period through precursor lesions which can be detected by electrochemical impedance sensor. The HPV driven molecular mechanisms underlying the development of cervical lesions have provided a number of potential biomarkers for both diagnostic and prognostic use in the clinical management of women with HPV related cervical disease and these biomarkers can also be used to increase the positive predictive value of current methods. The most influential methods for the detection and identification of HPV using gold nanoparticle (GNP) included electrochemical impedance sensor will visit their sensitivity, selectivity and characteristic detection on synthetic target which are complement of the DNA, non-complement of the DNA and mismatch of the DNA. In difference concentration of synthetic target, which stage can get the exactly value to determine the HPV in strain 16 was evaluated in this research studies.
  • Publication
    Voltammetric DNA Biosensor for Human Papillomavirus (HPV) Strain 18 Detection
    ( 2020-07-09)
    Mhd Akhir M.A.
    ;
    Nor Azizah Parmin
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    ;
    Voon Chun Hong
    This research was developed to focus on the study of the voltammetric DNA biosensor for the detection of HPV strain 18. In this research, electrical DNA biosensor was expected to detect HPV strain 18 more efficiently by using electrical characterization. In this project, device inspection was conducted to make sure the functional of the gold interdigitated electrode (IDE) by using Scanning Electron Microscope (SEM). 3-Aminopropyl Triethoxysilane (APTES) solution was used for the process of surface modification to form the amine group on the surface of the device to facilitate the attachment of the DNA probe. In this project, synthetic DNA sample and DNA from the saliva of several Biosystems Engineering students were used as the target DNA. The current-voltage (I-V) electrical characterization was conducted to detect the presence of HPV strain 18 in both DNA samples. As the results, perfect alignment between the electrodes on the IDE was detected under SEM. Surface modification of the biosensor successfully conducted which is the covalent bond between APTES and DNA probe increase the electrical. Synthetic DNA shows the presence of HPV strain 18 while there was no HPV strain 18 detected in the DNA from saliva samples.
  • Publication
    Designing DNA probe from human Papillomavirus (HPV) 58 in E6 region as biosensing element for development of biosensor
    ( 2024-03-21)
    Jaapar N.F.
    ;
    Nor Azizah Parmin
    ;
    Halim N.H.A.
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Ruslinda A. Rahim
    ;
    Nadzirah S.
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Ang W.C.
    ;
    Zakaria I.I.
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Hamzah A.A.
    ;
    Dee C.F.
    ;
    Halim F.S.
    Globally, second leading cause of death for women is a Cervical Cancer. CC is caused by infection of Human Papillomavirus (HPV). HPV strains 16 (50.8%), 18 (17.6%), and 58 (2.6%) became the most leading strains of infection in Malaysia. Recently, a study showed that HPV 58 was rare worldwide but famous in Asia countries including Malaysia. However, detection the significance of HPV-58 in women has not been studied extensively because of rare case compared to HPV 16 and 18. HPV-58 is commonly found in East Asia, but infrequently worldwide, due to changes in the environment of viruses and humans. Detailed biological knowledge is crucial for the development of effective countermeasures, diagnostic tests, vaccines and antiviral drugs against the HPV. The oligonucleotide sequences of HPV 58 in E6 region have been analysed between 24-35 mer in order to maintain the specificity and selectivity. The percentage of similarities between the coding sequences has developed with 66.7% of GC content. The DNA probe of HPV 58 was 5'GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3' with 30 mer of oligonucleotides. The important of E6 region for developing the coding sequence as it involved in the DNA reproduction, transcription, translation regulation and transformation of HPV genome. Phylogenetic trees were then constructed by Neighbour-Joining and the Kimura 2-parameters methods, followed by an analysis of selection pressures acting on the E6/E7 genes by ebi ac uk tools.
  • Publication
    Design and Fabrication of Multichannel PDMS Microfluidic
    ( 2021-12-14)
    Muhammad Nur Aiman Uda
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Thivina V.
    Microfluidic delivers miniaturized fluidic networks for processing liquids in the microliter range. In the recent years, lab-on-chip (LOC) is become a main tool for point-of-care (POC) diagnostic especially in the medical field. In this paper, we presented a design and fabrication on multi disease analysis using single chip via delivery of fluid with the multiple transducers is the pathway of multi-channel microfluidic based LOC's. 3 in 1 nano biosensor kit was attached with the microfluidic to produce nano-biolab-on-chip (NBLOC). The multi channels microfluidic chip was designed including the micro channels, one inlet, three outlet and sensor contact area. The microfluidic chip was designed to include multiplex detection for pathogen that consists of multiple channels of simultaneous results. The LOC system was designed using Design Spark Mechanical software and PDMS was used as a medium of the microfluidic. The microfluidic mold and PDMS microfluidic morphological properties have been characterized by using low power microscope (LPM), high power microscope (HPM) and surface profiler. The LOC system physical was experimental by dropping food coloring through the inlet and collecting at the sensor contact area outlet.
  • Publication
    Novelty Studies on Amorphous Silica Nanoparticle Production from Rice Straw Ash
    ( 2020-07-09)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Halim N.H.
    ;
    Anbu P.
    Turning waste product into the valuable resources is the best alternative way to overcome the waste management issue. Generally, rice is grown and planted twice a year where a lot of rice by-products have been produced after harvesting the matured paddy. Rice straw is one of turning waste products into the valuable resources and to manage the environmental issues. Generally, rice is grown and planted twice a year where a lot of rice by-products are produced. Rices straw is one of the rice by-products, generated roughly 0.7-1.4 kg per kilograms of harvested milled rice. With the nanotechnological approach, silica particles at nano-size can be produced using the incinerated rice straw. In addition to that, this research will report the synthesis, characterization and adsorption analysis towards the heavy metal removal.
  • Publication
    Morphological Analysis of Fabricated 5.0 μM Interdigitated Electrode (IDE)
    ( 2021-12-14)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Halim N.H.
    ;
    Hashim M.K.R.
    ;
    Anbu P.
    The aim of this research is to study the morphological analysis of fabricated Interdigitated Electrode (IDE). This device electrode was physically characterized using 3D nano profiler, scanning electrode microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Atomic Force Microscope (AFM). Based on this analysis, IDE pattern was analyzed thoroughly based on the IDE pattern specifications with 5 μM finger gap and this research significantly will stand as a platform quantify the biomolecules in further analysis.
  • Publication
    Label-free aptamer based biosensor for heavy metal detection
    ( 2020-11-02)
    Muhammad Nur Aiman Uda
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Isa A.M.
    Heavy metal contamination such as arsenic becomes serious threat to the health and environment of many millions in a worldwide. Arsenic contamination especially in paddy field has driven to the accumulation of As in paddy soils and possibly have adverse effects on rice products. To assess the level of severity of arsenic in paddy field is a major constraint at early stages. In the past, quantification of concentration of arsenic has been as difficult as reducing it. Thus label free Aptamer based biosensor is the most reliable sensor recently due to the advantages for the sensitive and selective detection towards detecting arsenic. Finally, this paper will discuss the current outcomes in arsenic detection using Aptasensor with sensing element formatting in enhancing the sensor signal for detection.
  • Publication
    Simple and Green Approach Strategy to Synthesis Graphene Using Rice Straw Ash
    ( 2020-07-09)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    ;
    Nur Hulwani Ibrahim
    ;
    Nor Azizah Parmin
    ;
    Halim N.H.
    ;
    Anbu P.
    Abundant resources of rice straw and carbon-neutral have a lot of potentials to be converted into various value-added product and become more green due to less utilization of chemical usage. Graphene is one of value-added had been converted from rice straw and was successfully synthesized by using activating rice straw ash (RSA) using potassium hydroxide (KOH) at 700 oC with 1:2.5 impregnation ratio. scanning electron microscopy (SEM), field emission scanning electron microscopy (FESEM), EDX Analysis with a field emission transmission electron Microscope (FETEM) and atomic force microscopy (AFM). The current results can be the model for comparing the synthesize graphene from other plant sources.
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  • Publication
    Evaluation and Optimization of Genomic DNA Extraction from Food Sample for Microfluidic Purpose
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Shaharuddin S.N.A.
    Contamination of various food samples became one of the critical issues in food pathogen infection. Food pathogen can be detected by using digital polymerase chain reaction (PCR) and sequencing. These methods were reliable but consuming and take a longer time for detection. The present work describes the innovation to develop a technology to extract double-stranded deoxyribonucleic acid (dsDNA) from food samples and then denatured dsDNA into and single-strand DNA (ssDNA) for further use on the chip using microfluidic device. Microfluidic device is a lab-on-chip device that consist of microfluidic channels that provide paths for biomolecules to flow to individual point of care. DNA extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. Lysis solution is used in the process of extraction the DNA to break up the cells containing DNA from protein and other cellular materials. This extraction firstly be done in the most labour-intensive in obtaining the DNA biomolecules. Extraction methods may require an overnight incubation, may be a protocol that can be completed in minutes or a couple of hours by using a commercial kit. The disadvantages of the laboratory and commercial kit is due to time-consuming, poor cost-effectiveness, the need to use big laboratory and a complicated process which need an expertise to conduct the experiment and interpret the data. This research is proposed to design and fabricate a microfluidic device that has DNA extraction capabilities. In this research DNA extraction using a commercial kit will be used as a comparison for the quality of the result. The microfluidic device can be used in health care delivery system and will help the doctors in diagnostic process to identify disease of a patient rapidly. Other than that, the output extracted from microfluidic device will be used for DNA probe target interaction for diagnostic kit. The major advantage of microfluidic device is that it consumes less time compared to the conventional chemical methods.