Nur Hamidah Abdul Halim
Thumbnail Image
Preferred name
Nur Hamidah Abdul Halim
Official Name
Nur Hamidah, Abdul Halim
Alternative Name
Halim, Nur Hamidah Abdul
Halim, N. H.A.
Hamidah A Halim, N.
Halim, N. H.
Halim, N. Hamidah A.
Abdul Halim, Nur Hamidah
Main Affiliation
Scopus Author ID
55493444600

Research Output

Filters

4
16
Reset filters

Settings
Now showing 1 - 10 of 16
  • Publication
    Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
    ( 2022-10-01)
    Jaapar F.N.
    ;
    Nor Azizah Parmin
    ;
    Nur Hamidah Abdul Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Halim F.S.
    ;
    Ruslinda A. Rahim
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    ;
    Nadzirah S.
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Zakaria I.I.
    The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high-end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross-validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5′ COOH-GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3′, while 5′COOH-GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3′ as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini-size diagnostic devices for cervical cancer detection.
  • Publication
    Cost effective negative Plenum Cleanroom for Microelectronic Engineering undergraduate
    ( 2005)
    Uda Hashim
    ;
    Zul Azhar Zahid Jamal
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Nur Hamidah Abdul Halim
    he Negative Plenum Cleanroom which is design and built by KUKUM is primarily used for the teaching of the undergraduate microelectronic course. The cleanroom is approximately 115m² in size. The level of cleanliness in the cleanroom ranges from ISO Class 5 (Yellow Room) to ISO Class 8 (Grey Area/Utility Chase). The cleanroom is constructed with a negative plenum to house the fan filter units, which make it different from other commercially available cleanrooms. With negative plenum, maintenance work cost will be reduced and make the cleanroom life longer. The main intention of this project is to expose and teach students to appreciate the stringent cleanroom protocols, health and safety requirement in addition to the formal course works.
  • Publication
    Electrochemiluminescence of carbon dots and nitrogen-doped carbon dots from a microwave-assisted method
    ( 2023-10)
    Nurul Izzati Akmal Mohd Azman
    ;
    Muhammad Amirul Afiq Abdul Halim
    ;
    Nur Syakimah Ismail
    ;
    Nur Hamidah Abdul Halim
    ;
    Nurjuliana Juhari
    ;
    Norhayati Sabani
    ;
    Siti Aisyah Shamsudin
    ;
    Eiichi Tamiya
    This research focuses on the use of carbon dots (CDs) and nitrogen-doped carbon dots (NCDs) synthesized using a microwave-assisted method as electrochemiluminescence (ECL) luminophores. CDs have been synthesized using citric acid, while various concentrations of nitrogen-doped CDs have been successfully obtained by varying the amount of urea from 1 to 3 g with citric acid to produce NCD1, NCD,2 and NCD3. The ECL mechanism of CDs and NCDs on screen-printed electrodes has been studied using cyclic voltammetry (CV). ECL emission from as-prepared CDs and NCDs was observed in PBS with potassium persulfate (K2S2O8) as a co-reactant. The addition of potassium chloride (KCl) as a supporting electrolyte displays fast electroreduction of CDs and K2S2O8 to expedite the generation of CDs and peroxydisulfate radicals that simultaneously increase ECL intensity. Furthermore, as the concentration of nitrogen-doped CDs increases, so does the intensity of the ECL. NCD3 shows the highest ECL intensity by an increment of 86.4% in comparison to CDs in PBS with the addition of K2S2O8 and KCl. Finally, optimization of ECL measurement was carried out in terms of CV potential range, concentration of luminophore, supporting electrolyte, and co-reactant using NCD3 luminophore. The CV potential range at 0 to -2 V shows 50 mV of early CV reverse onset potential that resulted in an increase of 52.9% ECL intensity. Meanwhile, 30x dilution of NCD3, 0.1 M of supporting electrolyte KCl, and 0.1 M of co-reactant K2S2O8 show the optimum value to obtain high ECL intensity.
  • Publication
    Effect of heating power towards synthesis of carbon dots through microwave pyrolysis method for optical-based biosensor
    ( 2020-01-08)
    Nur Syakimah Ismail
    ;
    Husain, Umi Shahirah
    ;
    Selvan S.
    ;
    Mordani, Nor Afnia
    ;
    Nurjuliana Juhari
    ;
    Nur Hamidah Abdul Halim
    This study focuses on the effect of heating power in producing carbon dots (CDs) using microwave-assisted pyrolysis from glucose and PEG-200. Glucose is one type of saccharide that contains carbon atom in their structure while PEG-200 is a polymer that acts as a stabilizer. Synthesis of CDs was carried out by using a domestic microwave at different heating power ranging from 300 to 600 Watts. Produced CDs were subjected to dilution, filtering and dialysis procedure for purification purpose. UV-Vis was carried out using PerkinElmer Lambda 35 Spectrophotometer to prove the present of carbon dots in the sample solution. Photoluminescence intensity was observed using Horiba FluoroLog Spectrophotometer together with Horiba iHR550 Imaging Spectrometer to show the emission wavelength and the emission color of CDs prepared. UV-Vis results show the present of CDs in each sample from absorption peaks in the range between 240 nm until 260 nm. The photoluminescence testing shows the emission wavelength of prepared CDs solution is around 430 nm to 570 nm which represent emission color of green-yellow luminescence. From this experiment, CDs that were prepared using a heating power of 450 Watt in 2 minutes and went through the filtering process produce the best luminescence properties. Prepared CDs display electrochemiluminescence (ECL) properties in the presence of co-reactant potassium peroxydisulfate using cyclic voltammetry to pave the way for optical-based biosensor application.
  • Publication
    Electrochemical detection of heavy metal ions using gold nanoparticles on carbon dots extracted from curry leaves
    ( 2024-06)
    Nur Syakimah Ismail
    ;
    Aidil Safiy Kamarul Ariffin
    ;
    Nurul Izzati Akmal Mohd Azman
    ;
    Nur Hamidah Abdul Halim
    ;
    Norhayati Sabani
    ;
    Nurjuliana Juhari
    ;
    Siti Aisyah Shamsudin
    Carbon dots (CDs) have attracted attention due to their versatility in electronic and optical properties based on precursor and type of synthesis process. Recently, many researchers have focused on using natural resources or wastes to form CDs. Four samples of CDs have been synthesized from curry leaves using a microwave-assisted approach at heating powers of 700 and 800 V with durations of 5 and 8 minutes. UV-Vis and FTIR spectra reveal the existence of carbon graphitic elements with carboxyl and hydroxyl functional groups on the surfaces of CDs. CVs of AuNPs/CDs/GS electrodes in ferricyanide disclosed that as-synthesized CDs produced using a lower heating power of 700 W exhibit pronounced electrocatalytic activity with sluggish electron transfer kinetics. Conversely, as-synthesized CDs created with a higher heating power of 800 W demonstrate reduced electrocatalytic activity but rapid electron transfer kinetics. Electrochemical detection of Pb2+ ions was observed through a sharp peak around -0.42 to -0.438 V, while detection of Hg2+ ions was observed through two anodic peaks around +0.334 to +0.408 V during a forward scan in acetate buffer (pH 4.5) on AuNPs/CDs/GS electrodes when tested individually. These distinct peaks also appeared in mixture solutions, with a slight reduction in peak current density that suggests the selectivity of the AuNPs/CDs/GS electrodes towards Pb2+ and Hg2+ ion detection. The optimum AuNPs/CDs/GS electrode for sensitive and selective detection of Pb2+ and Hg2+ was recorded using CDs D as a functional supporting matrix for AuNPs that was synthesized using a heating power of 800 W for 8 minutes.
  • Publication
    Silicon nanowire biosensors for diabetes mellitus monitoring
    ( 2024-10)
    M. Shaifullah A. S
    ;
    J. Jumat
    ;
    Mohammad Nuzaihan Md Nor
    ;
    J. N. Ismail
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Nur Hamidah Abdul Halim
    ;
    Zarimawaty Zailan
    ;
    Mohd Khairuddin Md Arshad
    ;
    M. Syamsul
    ;
    Rozaimah A. T
    The main goal of this research is the development of a label-free biosensor for the detection of diabetes mellitus (DM) using the target molecule retinol-binding protein 4 (RBP4). The enzyme-linked immunosorbent assay (ELISA) approach, currently used to detect DM, is time-consuming and difficult. As a result, label-free biosensors are being considered as an alternative. In this research, silicon nanowires (SiNWs) were selected as the transducer for this biosensor due to their low cost, real-time analysis capability, high sensitivity, and low detection limit. The SiNWs were created using conventional lithography, reactive ion etching (RIE), and physical vapor deposition (PVD), and then dripped with a gold nanoparticle solution to create gold-decorated SiNWs. The surface of the gold-decorated SiNWs was functionalized using 3-aminothiophenol and glutaraldehyde solutions before being immobilized with DM RBP4 antibodies and targets. The electrical characterization of the gold nanoparticle decorated SiNWs biosensor revealed good performance in DM detection. The pH tests confirmed that the SiNWs acted as a transducer, with current proportional to the DM RBP4 concentration. The estimated limit of detection (LOD) and sensitivity for detecting DM RBP4 binding were 0.076 fg/mL and 8.92 nA(g/mL)-1, respectively. This gold nanoparticle decorated SiNWs biosensor performed better than other methods and enabled efficient, accurate, and direct detection of DM. The SiNWs could be used as a distinctive electrical protein biosensor for biological diagnostic purposes. In conclusion, gold nanoparticle deposition offers effective label-free, direct, and high-accuracy DM detection, outperforming previous approaches. Thus, these SiNWs serve as novel electrical protein biosensors for future biological diagnostic applications.
  • Publication
    Impact of Nanogap Thickness on Dielectric-Modulated Field-effect Transistor Biosensor Performance for Uncharged Biomolecules Detection
    ( 2023-01-01)
    Jasmi M.S.
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Mohd Khairuddin Md Arshad
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Nur Hamidah Abdul Halim
    ;
    Rahman S.F.A.
    ;
    Shaifullah. A. S M.
    ;
    Adilah Ayoib
    ;
    Ibrahim M.M.
    Uncharged biomolecules sensing performance of dielectric-modulated field-effect transistor (DMFET) biosensor at various nanogap thickness via semiconductor device simulation tool was assessed in this work. The device structures with 10 nm-, 15 nm-, and 20 nm-thick nanogap were constructed for this investigation. Each device structure was applied with dielectric constant ranging from 2 to 7 at the nanogap representing the presence of various biomolecules. These device structures were electrically simulated by supplying gate voltage from 0 V to 2 V and biased with drain voltage of 0.05 V for linear region of operation. Based on the extracted drain current, the reduction of nanogap thickness increase capacitance at the nanogap region. In additional, increase in nanogap's dielectric constant causing an increase of its capacitance, and translated into higher output drain current. Sensitivity calculation and analysis shows DMFET biosensor with 10 nm-thick nanogap demonstrated the highest sensitivity with 6.896 μA/dec, which possibly permit enhanced sensing of uncharged biomolecule.
  • Publication
    Fabrication and simulation of silicon nanowire pH sensor for Diabetes Mellitus detection
    ( 2023-04)
    C. Y. Chean
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Mohamad Faris Mohamad Fathil
    ;
    M. I. Hashim
    ;
    Nur Hamidah Abdul Halim
    ;
    Zarimawaty Zailan
    ;
    Mohd Khairuddin Md Arshad
    ;
    Syamsul Syahrun Awang@Hashim
    ;
    Rozaimah A.T
    Diabetes Mellitus (DM) is a disease failed to control the balance of blood sugar level due to lack of insulin thereby it effect human health. In Malaysia, there are around 3.9 millions people aged 18 years old and above have diabetes according to National Health and Morbidity Survey 2019. Silicon Nanowire is a nanostructure which has ultra-high sensitivity and non-radioactive that has potential given good performances when applied on pH sensor and biosensor. Silicon nanowire pH sensor and biosensor is an electronic sensor that investigated to improve the sensitivity and accuracy for detecting DM. This project consists of two parts, which are fabrication of silicon nanowire pH sensor and simulation of silicon nanowire biosensor as preliminary study. In fabrication, silicon nanowire of pH sensor is fabricated by conventional lithography process, reaction ion etching (RIE) and metallization to achieved the width of 100 nm silicon nanowire. The pH6, pH7, pH10 and DI water as analytes to analysis the current-voltage (I-V) characteristics of silicon nanowire pH sensor. In second part, the silicon nanowire biosensor as preliminary study is done simulation by Silvaco ATLAS devices simulator. The silicon nanowire with 30 nm in height and 20 nm in width of biosensor is designed and simulated to analyze the performance in terms of sensitivity. I-V characteristics of silicon nanowire biosensor according to different concentration of negative interface charge is determined. The negative interface charge represent as the Retinol Binding Protein 4 (RBP4) which is used to diagnose DM. The I-V characteristic based on the change in current, resistance and conductance to determine sensitivity. Lastly, the sensitivity of silicon nanowire pH sensor obtained 23.9 pS/pH while the sensitivity of simulated silicon nanowire biosensor obtained 3.91 nS/e.cm2. The results shown the more negative charge of concentration analyte attached on surface silicon nanowire has been accumulated more current flow from drain terminal to source terminal. It leads to the resistance becomes highest and obtained good sensitivity. In summary, the silicon nanowire pH sensor exhibited good performance and high sensitivity in detection pH level. The simulated silicon nanowire biosensor is capable of detecting biomolecular interactions charges to obtained high sensitive and accuracy result.
  • Publication
    Facile electrical DNA genosensor for human papillomavirus (HPV 58) for early detection of cervical cancer
    ( 2023-07)
    F. Nadhirah Jaapar
    ;
    Nor Azizah Parmin
    ;
    Nur Hamidah Abdul Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Ruslinda A. Rahim
    ;
    Sh. Nadzirah
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Wei Chern Ang
    ;
    Iffah Izzati Zakaria
    ;
    Zulida Rejali
    ;
    Amilia Afzan
    ;
    Azrul Azlan Hamzah
    ;
    Chang Fu Dee
    ;
    F. Syakirah Halim
    For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10μM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.
  • Publication
    Fabrication of Graphene Electrode via Graphene Transfer Method for Bisphenol A (BPA) Detection
    ( 2021-01-01)
    Shukri N.I.B.A.
    ;
    Norhayati Sabani
    ;
    Ruslinda A. Rahim
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Syarifah Norfaezah Sabki
    ;
    Nur Hamidah Abdul Halim
    ;
    Nur Syakimah Ismail
    Exposure of BPA is a concern as BPA can seep into food or beverages from containers and can possibly effects on human health especially endocrine systems. An electrochemical-based aptasensor utilizing graphene was developed in detecting endocrine disrupting compound Bisphenol A (BPA, 4,4'-(propane-2,2-diyl) diphenol). The graphene modified electrode was developed via graphene transfer. Fabrication and characterization of graphene transfer was studied in this paper using Scanning Electron Microscopy (SEM) and High-Power Microscope (HPM). In this research, the investigation of interfacial characteristic modified graphene with aptasensor and recognition of BPA with aptasensor had been done using electrochemical impedance spectroscopy (EIS). The increment of charge transfer resistance (Rct) before and after recognition of BPA denoting the accumulation of charge at the electrode surface in this research.